Subjects in all studies were 200-250 g, male Sprague-Dawley rats from Taconic Farms (Germantown, NY). They were individually housed in hanging wire cages and maintained on a 12:12-hr light-dark cycle with ad libitum access to LabDiet rodent chow (St. Louis, MO) unless otherwise stated. Water was always available, either through an automated system or through graduated cylinders attached to the cages. All animal procedures used in these studies were reviewed in advance by the Princeton University Institutional Animal Care and Use Committee and followed the NIH Guide for the Care and Use of Laboratory Animals. Adequate measures were taken to minimize animal pain and discomfort.
