At least 200 cells were imaged in phase contrast in each of 3 replicate wells of 2 isogenic lines of pMGLs (Rett and control). FiJi Gaussian blur was applied to the images, followed by background subtraction and thresholding. The particle counting function was used to measure average pixel counts (surface) of cell masks, and average perimeters, in each replicate. Data is given as mean ± s.e.m., with significance below 0.05 for the t-test.
