EdU (10μM, Life Technologies) was added to MGdM medium for 24 hours, after which cells or tissues were fixed using ice-cold methanol. EdU click-it assay was performed on fixed cells per the manufacturer’s instruction (Life Technologies), followed by fluorescent immuno-staining and imaging. Counting was performed by automated particle counting with Fiji. Data is represented as a ratio of EDU positive to DAPI positive nuclei. 6 random fields were chosen at 100x magnification, and averaged between two biological replicates to generate s.e.m. and t-test values.
