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Chunk #30 — Methods — Duke Neurogenetics Study (DNS; N = 312)

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Monoacylglycerol lipase (MGLL) polymorphism rs604300 interacts with childhood adversity to predict cannabis dependence symptoms and amygdala habituation: Evidence from an endocannabinoid system-level analysis.
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electronic Mini International Neuropsychiatric Interview (Sheehan, 1998) and Structured Clinical Interview for the DSM-IV Axis II (SCID-II; First, Gibbon, Spitzer, Williams, & Benjamin, 1997). These disorders are not exclusionary, as the DNS seeks to establish broad variability in multiple behavioral phenotypes related to psychopathology. Participants were excluded (n = 13) for quality issues in data collection: a large number of movement outliers in fMRI data (n = 3), inadequate signal in our regions of interest (n = 6), and poor behavioral performance (n = 4). This resulted in a final sample for analysis of 312 (Table 3). Details regarding genotyping are available in the Supplement. The top SNP from CATS, rs604300 in MGLL (call rate = 100%, HWE p = 0.75, MAF = 0.10), was the only variant examined for the purposes of these analyses; due to its low minor allele frequency and the resulting small number of minor homozygotes in DNS (n = 2), AA/AG participants were combined to form a minor allele carrier group (n = 61).