Chunk #20 — Results — Identification of a potent locus associated with human opioid sensitivity by GWAS in subjects who underwent painful cosmetic surgery
For further fine mapping of this region, we used the remainder of the genotyped data and imputed the genotype data from the SNPs that spanned 300 kbp, including both the METTL21A and CREB1 genes (Figure 1a), and analyzed the association. We then identified several other SNPs around the best candidate SNP, rs2952768, with nominal P-values from 1 × 10−6 to 1 × 10−4 (Figure 1b). After the LD analysis, all of these SNPs were found to be included in an LD block that spanned approximately 134 kbp and were in strong LD (D′=1, r2>0.8) with rs2952768 (Figure 1c). Total fentanyl use during the 24-h postoperative period was 1.080±0.053, 1.146±0.049 and 1.666±0.083 μg kg–1 (log transformed; mean± s.e.m.) in subjects with the T/T, T/C and C/C genotypes, respectively (Figure 2a).
