To investigate enrichment of biological pathways, we analyzed 8,664 gene-sets derived from GO Biological Process (N=7,658) and GO Cellular Components (N= 1,006) ontology in the MSigDB database, identifying 479 significant gene-sets after correction for multiple testing (FDR<0.05, Supplementary Table S8), which included several gene-sets that have not previously shown significant enrichment in depression14,25. The majority of the top-ranking sets were related to neuronal development and function, including the five most significant gene-sets (Padj<10−14): GO_SYNAPSE, GO_NEURON PART, GO_SYNAPSE ORGANIZATION, GO_SYNAPSE ASSEMBLY and GO_NEURON DIFFERENTIATION.
