Chunk #54 — STAR*METHODS — METHOD DETAILS — Primary human hepatocytes

Source

Large, Diverse Population Cohorts of hiPSCs and Derived Hepatocyte-like Cells Reveal Functional Genetic Variation at Blood Lipid-Associated Loci.

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HEPES)] (Thermo Fisher Scientific). Each well was refed with 500 μL maintenance medium daily for the next 2 days. ApoB, triglyceride, and albumin secreted into the media by HLCs were measured as described above. For RNA-seq analyses, primary human hepatocytes from each vial were thawed and spun down as described above and then immediately processed for RNA isolation as described below.