Single vials containing 5 to 15 million viable primary human hepatocytes each from lots derived from individuals were obtained from Thermo Fisher Scientific (Human Plateable Hepatocytes, Metabolism Qualified). All were rated to survive in culture for at least three days after plating. The cells from each vial were thawed into Cryopreserved Hepatocyte Recovery Medium (Thermo Fisher Scientific), gently spun down, and then resuspended in William’s Medium E with added Hepatocyte Plating Supplement (fetal bovine serum, dexamethasone, and a cocktail solution of penicillin-streptomycin, bovine insulin, GlutaMAX, and HEPES) (Thermo Fisher Scientific) and plated at 400,000 cells/well in collagen-coated 24-well plates, according to the manufacturer’s instructions. After 6 hours, each well was refed with 500 μL maintenance medium [William’s Medium E with added Hepatocyte Maintenance Supplement (dexamethasone and a cocktail solution of penicillin-streptomycin, insulin, transferrin, selenium complex, BSA, linoleic acid, GlutaMAX and HEPES)] (Thermo Fisher Scientific). Each well was refed with 500 μL maintenance medium daily for the next 2 days. ApoB, triglyceride, and albumin secreted into the media by HLCs were measured as described above. For RNA-seq analyses, primary human hepatocytes
