Figure 4 shows the distributions of correlation coefficient of male and female group of the same channel. The results of quantitative correlation analysis of ΔHbO2, ΔHb and ΔtHb indicated strong linear correlation between male and female group with R2 = 0.6342, p < 0.0001; R2 = 0.3038, p < 0.0001; and R2 = 0.6182, p < 0.0001, respectively. The results suggest the fOT-based brain functional connectivity matrices are reliable across different groups. This allowed us to further analyze the brain functional connectivity of gender effects between male and female groups.