The 13 genotyped CRHR1 SNPs included those from prior reports (Blomeyer et al. 2008; Treutlein et al. 2006; Bradley et al. 2008) and nearby SNPs identified from dbSNP. HWE p values were >0.1 for all SNPs; no evidence of substantial Mendelian errors was found. The call rate for rs3029044, an insertion/deletion polymorphism, was 94%; call rates for the SNPs otherwise ranged from 0.95 -0.98 (TABLE 1). We examined the linkage disequilibrium (LD) relationships of the 13 SNPs (shown in FIGURE 1 as pairwise r2 values) and recognized that the large block of 8 SNPs (including rs1876831) in very strong LD with minor allele frequencies (MAFs) ranging from 0.21 to 0.22 (TABLE 1) are part of the H2 haplotype. The other 5 SNPs forming two small additional LD blocks apart from the H2 haplotype are not included in the current analyses. Data were coded consistent with the prior report (Blomeyer et al. 2008) to enable comparison of individuals with one or more copy of the H2 haplotype to individuals homozygous for the H1 haplotype (ie, consistent with a dominant mode of inheritance).
