Chunk #2 — Results — In vitro neuronal progenitor cell model

Source

Characteristic analyses of a neural differentiation model from iPSC-derived neuron according to morphology, physiology, and global gene expression pattern.

Embedded

yes

Text

We first established an in vitro neuronal progenitor cell (NPC) model by culturing hiPSCs with a two-inhibitor culture system. At the end of the culture period, the treated hiPSCs were stained for neuroectodermal stem cell markers including NESTIN, PAX6, and SOX2. We found that the majority of the treated cells stained positive for these markers, indicating that most of the treated hiPSCs differentiated into NPCs (Fig. 1).