Following these analyses, we genotyped the top 100 polymorphisms in a further independent Munich cohort of 298 schizophrenic patients and 713 healthy controls, all self-identifying as of German or central European ancestry. Using the Sequenom iPLEX system, we successfully genotyped 98 of the 100 SNPs and found that 8 of these 98 variants showed an association that was significant at the 0.05 level in the independent cohort (rs2135551, rs950169, rs1911155, rs4745431, rs4745430, rs4487082, rs3748376 and rs11635597). These included the most strongly associated three SNPs in the list: rs2135551, rs950169 and rs1911155 in ADAMTSL3 (in linkage disequilibrium with one another) (Table 1). Since 3 of these 8 SNPs are in strong LD, this is approximately the number of significant associations we would expect by chance at p<0.05, however in all 8 cases the direction of effect was the same as in the original cohort. The combined p value for the strongest associated SNP (rs2135551) across the original and first replication studies is 1.3×10−7. If we use a Bonferroni correction for all the SNPs considered in this study (312,565 SNPs that passed
