This test was used to assess acquisition and memory of spatial learning. The apparatus was a large, circular pool (diameter = 122cm; 45cm deep), filled with water (24–26°C) and surrounded by four discrete extra-maze cues effectively dividing the apparatus into quadrants. The water was made opaque with nontoxic poster paint. A camera suspended above the maze was connected to Noldus Ethovision (Ethovision, Noldus Information Technology, Wageningen, the Netherlands). Dependent measures included swimming distance, velocity, latency to find the platform, time spent in each quadrant, path efficiency to the platform.
