Rotarod was used to assess graded motor coordination and balance in mice over 3 repeated trials. Each trial was a maximum of a 5-min test and there was a brief inter-trial interval (45s). Mice were placed on a rotating barrel (3cm) of a rotarod apparatus (Ugo-Basile, Stoelting Co., Wood Dale, IL) which progressively accelerated from 3rpm to 30rpm. The latency to fall off or rotate around the top of the barrel was recorded as the primary measure of neuromuscular coordination.
