To transfect the ReN cells with the lentiviral constructs, 50–100 μl viral solution (1 × 106 TU/ml) were added to 85% confluent proliferating ReN cells in 6-well dishes, incubated for 24 hours, and washed 3 times to stop the infection. The expression of the infected genes was confirmed by mCherry or GFP expression by fluorescence microscopy and WB analysis.
