We also attempted to replicate a reported enrichment of association signals for two gene sets that comprised high confidence targets of two miRNA families (see the Stewart et al OCD GWAS). Analyses of the miRNA families of predicted miR-130ac/301ab/301b/301b-3p/454/721/4295/3666 (miRNA set 1) and miRNA-219-5p/508/508-3p/4782-3p (miRNA set 2) targets showed nominal significance for 3 out of 45 high confidence targets in miRNA set 1 and 14 out of 145 high confidence targets in miRNA set 2. The number of nominal significant genes for both sets are higher then expected by chance; however, for both sets, no significant enrichment for OCD associated genes was found in either set (one-sided P=0.323 and one-sided P=0.087, respectively). It is of note that one of the high confident targets in miRNA set 2 (the epiregulin gene, EREG) did demonstrate an experiment-wise significance level (correcting for the number of tests performed in miRNA set2; P=3.06×10−4; Pcorr=0.044).
