All testing was performed on animals between the ages of 9 to 14 weeks. Mice were tested for loss of righting reflex as a function of dose following injections of ethanol (3.2, 3.6 and 4.0 g/kg, i.p.). The same animal was tested at all three doses, separated by two weeks between each test. Dose order was counterbalanced. After injection, animals were intermittently placed on their backs and tested for loss of righting reflex. Loss of righting reflex was defined as the inability to right itself three times within a 30 second interval. Duration was defined as the time interval between the loss and return of the righting reflex.
