The NCBI small eukaryotic genome pipeline is a new automated pipeline designed for the generation of RefSeq records as a result of direct propagation of annotated INSDC records. The RefSeq records thus generated are copies of the GenBank data with some format changes to adhere to RefSeq requirements. The most notable difference between the original INSDC record and the RefSeq record is the addition of the RefSeq transcript product. Although not designed to generate de novo genome annotation, the small eukaryotic genome pipeline draws from several of the NCBI eukaryotic genome annotation pipeline modules and their code (http://www.ncbi.nlm.nih.gov/books/NBK169439/).