Aldh2−/−Fancd2−/− mice were generated on a C57BL/6 × 129S4S6/Sv F1 background. To this end, the previously reported Fancd2 allele (Fancd2tm1Hou; MGI ID: 2673422, a gift from M. Grompe) was back-crossed onto the C57BL/6Jo1a background for 10 generations and crossed with Aldh2+/− (C57BL/6N) mice to generate Aldh2+/−Fanca+/− mice on a pure C57BL/6 background. Likewise, the previously reported Aldh2 allele (Aldh2tm1a(EUCOMM)Wtsi; MGI ID: 4431566, EUCOMM6) was backcrossed from C57BL/6N onto 129S6/Sv for five generations and crossed with Fancd2+/− mice to generate Aldh2+/−Fancd2+/− mice on a 129S4S6/Sv background. Finally, Aldh2−/−Fancd2−/− and control mice were generated as F1 hybrids from crosses between Aldh2+/−Fancd2+/− females (129S4S6/Sv) and Aldh2+/−Fancd2+/− males (C57BL/6).
