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Chunk #10 — Methods — Electrophysiology

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Alcohol reverses the effects of KCNJ6 (GIRK2) noncoding variants on excitability of human glutamatergic neurons.
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Analyses of iN used whole-cell patch-clamp electrophysiology as previously described [24, 25] with details provided in the Supplementary Methods. Drugs were applied through a perfusion system in the following concentrations: 160 nM ML297 or 20 mM ethanol. Clampfit software (pClamp 11; Molecular Devices) was used for analysis of recordings.