For bulk RNAseq, individual iN cultures were harvested in lysis buffer and column purified (Quick-RNA kit, Zymo). RNA was sent to Novogene, Inc., for sequencing. Analysis is described in the Supplementary Methods. RNAseq data have been deposited with the NIH GEO repository (accession number GSE196491). For single-cell RNA sequencing (scRNAseq), iN cultures were treated for 7 days using IEE [25] starting 21 days after plating onto glia. At 28 days, iN were dissociated using trypLE Express (Thermo Fisher) for 5 min at 37 °C and then processed and analyzed as described in the Supplementary Methods. These data have been deposited with the NIH GEO archive, accession number GSE203530.
