Chunk #12 — Results — Transcript levels in CB1R heterozygous and knockout mice

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Cortical glutamic acid decarboxylase 67 deficiency results in lower cannabinoid 1 receptor messenger RNA expression: implications for schizophrenia.

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Manipulation of the Cnr1 gene produced the expected gene dose-dependent effect (F1,15=381.6; p<0.001) on CB1R mRNA expression (Figure 4A–D). Post-hoc analysis demonstrated that mean CB1R mRNA levels were significantly 49.6% lower in CB1R+/− mice (210.9 ± 37.2) compared to WT mice (418.9 ± 21.7) and 94.6% lower in CB1R−/− mice (22.6 ± 2.2). However, neither GAD67 (F1,15=0.4; p=0.653; Figure 4E–H) nor DAGLα (F1,15=2.4; p=0.126; Figure 3I–L) mRNA levels were altered in CB1R+/− or CB1R−/− mice relative to WT mice.