Standard blood samples were obtained from the Johns Hopkins A-T Clinic, with approval from the Johns Hopkins IRB (#NA_00077304), and shipped to RUCDR Infinite Biologics. Reprogramming from activated T cells using Sendai viral vectors expressing Oct4, Sox2, Klf4, and Myc (Cytotune; Life Technologies) followed protocols described previously (Moore et al., 2012). All iPSC cultures are grown under low (4%) O2 conditions (Guo et al., 2013). Procedures at Rutgers were confirmed as exempt by the Rutgers institutional review board (#E13-825). Individual “sublines” were picked from a single, primary colony of iPSC.
