RNA was prepared from frozen iPSC pellets using Trizol (Life Technologies). RNA-seq was performed at RUCDR Infinite Biologics using Illumina technology and analyzed as described previously (Kraushar et al., 2014). The accession number for the A-T cell line RNA-seq experiments reported in this paper is GEO: GSE71878. The RNA samples used for comparison in Figure 1B were obtained from a previous study (NSC; Sauvageau et al., 2013) or from unpublished results from our lab.
