Chunk #35 — Experimental Procedures — Immunocytochemistry

Source

Spontaneous ATM Gene Reversion in A-T iPSC to Produce an Isogenic Cell Line.

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Cultured cells were fixed in 4% paraformaldehyde. Primary antibodies were obtained from EMD Millipore (Oct-4 [clone 10H11.2], TRA-1-60 [MAB4360], γH2A.X [phospho-Ser139]) or from Abcam (P-ATMs1981 [ab36810]). Imaging was performed on an IN CELL 6000 automated confocal microscope system (GE Biosciences).