peer-reviewed literature. Second, the differential expression analysis results (including FC and P values) of differentially expressed mRNAs (absolute FC ≥ 1.3 and P < 0.05) identified in the same brain region were added, and the Expression Pairing function of the IPA miRNA Target Filter was applied to obtain miRNA–mRNA pairs in which their expression levels were negatively-correlated (i.e., upregulated miRNA–downregulated mRNA pairs or downregulated miRNA–upregulated mRNA pairs). Third, the obtained miRNA–mRNA pairs were used to construct miRNA–mRNA interaction networks. Finally, AUD-related canonical pathways were added to miRNA–mRNA networks to display miRNA–mRNA-pathway relationships.
