By small RNA-seq analysis of the 192 selected Set 1 samples (from 8 brain regions), we identified 19 differentially expressed mature miRNAs (absolute FC > 2.0 & P < 0.05) in one or more brain regions (4 in AMY, 5 in CN, 3 in CRB, 3 in HIP, 2 in NAc, 8 in PFC, 5 in PUT, and 2 in VTA) of AUD subjects (Fig. 1 and Table S2). Two miRNAs were upregulated (>2-fold increase & P < 0.05) in multiple brain regions (miR-10a-5p: HIP and NAc; miR-144-3p: CN and PFC) of AUD subjects, while three other miRNAs were downregulated (>2-fold decrease & P < 0.05) in multiple brain regions (miR-122-5p: AMY, CN, CRB, and VTA; miR-412-5p: AMY, CN, CRB, PUT, and VTA; and miR-6868-3p: AMY, CN, CRB, PFC, and PUT) of AUD subjects.
