By Affymetrix miRNA 4.0 microarray analysis of the 96 selected Set 2 samples (from 6 brain regions), we identified 52 differentially expressed (absolute FC > 2.0 & P < 0.05) miRNAs in one or more brain regions (1 in AMY, 1 in CN, 3 in CRB, 4 in HIP, 44 in PFC, and 4 in PUT) of AUD subjects (Fig. S6 and Table S3). Same as above, miR-412-5p was downregulated (>2-fold decrease & P < 0.05) in five of the six brain regions (AMY, CN, CRB, HIP, and PUT) of AUD subjects.
