No other variants in the H2 haplotype were predicted to disrupt transcription factor binding sites for factors known to be involved in CRH transcriptional control. While another site (-2336 A>T) that was part of this haplotype predicted the creation of a YY1 site, gel shift assays indicated that DNA-protein interactions were not altered as a consequence of this variant (data not shown). There were neither non-synonymous SNPs nor variants predicted to alter exon-intron splicing or mRNA stability.
