and in order to maintain consistent data analysis across experiments, the averages of those three sessions were used as the initial test session values in the two-way ANOVA. The acceptability of this action was determined by conducting one-way repeated-measures ANOVAs for the FN-439 and aCSF-treated animals to evaluate whether there were any differences between the three sessions and if not, the average of the three was used. This course of action was taken instead of “pooling” the data because the within-subject component of the analysis results in the exclusion of data that is not represented by equal sample sizes. In addition, to protect against the possibility of committing a Type I error, a two-way ANOVA was also conducted that utilized the data from the single session prior to escalation instead of the averaged values. Post-hoc independent-sample t-tests were conducted on the ethanol consumption data (g/kg) from the escalation sessions in order to compare the FN-439 and aCSF-treated animals for ethanol consumption.
