The influence of the Lightweight mutation on ethanol preference and consumption was also investigated on a B6D2 F1 hybrid genetic background. In general, ethanol consumption was much lower on this genetic background versus C57BL/6J (Figure S3). Significant increases in both preference and consumption were observed at the 6% and 10% ethanol concentrations (P<0.01, post hoc Tukey test). When the congenic Control/+ mice were tested on a B6D2 F1 genetic background, no alterations were observed in ethanol preference or consumption (Figure S3).
