Chunk #15 — Methods — RNA immunoprecipitation and western blot analysis — RNA immunoprecipitation

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The long non-coding RNA NEAT1 is responsive to neuronal activity and is associated with hyperexcitability states.

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RNA immunoprecipitation was performed as previously described6. At least 3 independent experiments were performed to investigate potential interactions. KCNAB2 and KCNIP1 antibodies were purchased from LifeSpan Biosciences Inc. (WA, USA).