A caveat to our data are that because we did not a priori expect to see splicing differences, our sequencing data were not performed with long-read sequencing techniques. Nor were statistical considerations, such as k-means clustering of transcripts or weighting exon-exon junction reads, used to compare splicing across genes. However, our data do show that reducing gene expression of five known spliceosome associated proteins in the adult MB disrupts formation of ethanol-cue-induced memory. Thus, we believe that the alternative transcript usage in our data, is in part, due to splicing that occurs during memory formation.
