Briefly, the brain tissue was homogenized in CHAPSO and proteinase inhibitor (Sigma-Aldrich). The homogenate was centrifuged at 100,000 g for 1 hour. Protein concentrations from each sample were measured by BCA method (Pierce Biotechnology Inc., Rockford, IL, USA). The rabbit polyclonal anti-PSD-95 antiserum was purchased from Biosource Inc., (Camarillo, CA, USA), and the monoclonal anti-synaptophysin antibody was purchased from Millipore (Temecula, CA, USA). The anti-β-tubulin antibody E7-a1 was obtained from the Developmental Studies Hybridoma Bank.
