Microarray experiments were essentially carried out as previously described [14]. Briefly, RNA was extracted from pulverized gray matter from BA10 (dorsal aspect, frontal pole) using Trizol reagent (Invitrogen, Carlsbad, CA). The quality and quantity of sample RNA was evaluated by Bioanalyzer electrophoresis (Agilent, Palo Alto, CA). Eight µg total RNA from each sample was reverse transcribed into 33P-labeled individual cDNA samples, and divided for duplicate hybridizations to Mammalian Gene Collection (MGC) arrays containing 9216 cDNA clones from the MGC clone set [19]. The arrays were exposed to a low-energy phosphor screen (Molecular Dynamics, Sunnyvale, CA) for 5 days, the screen scanned (Phosphorimager 860, Molecular Dynamics), and pixel intensities quantified using ImageQuant (Molecular Dynamics).
