total volume of 50 μl with the following thermal program; 98°C for 30 sec, 12 PCR cycles of 10 sec at 98°C, 30 sec at 60°C, and 15 sec at 72°C, followed by at 72°C for 5 min and then kept at 4°C. Remaining PCR primers were removed twice by using 1.2 volumes of AMPure XP beads (Beckman Coulter). The resulting libraries were checked for size and concentration by BioAnalyzer (Agilent) using the High-Sensitivity DNA Kit (Agilent). Qualified sequencing libraries were loaded on the HiSeq2000 (Illumina) using the custom sequencing primer, 5’-CGA CAG GTT CAG AGT TCT ACA GTC CGA CGA TCG AAA-3’.
