washed three times with 50 mM Tris-HCl (pH 7.5), and protein was eluted with 2× Laemmli sample buffer and analyzed by SDS-PAGE and autoradiography as described previously (Ireton et al., 2002). Quantification was performed by densitometry using Image Gage software (Fujifilm Inc.). Arbitrary densitometry units were plotted with GraphPad Prism (GraphPad Software, Inc.) and adjusted for background. Biotinylation of total surface cadherin was performed as described above, but without the pulse-chase labeling.
