LZRS–mp120–Neo has been described in detail previously (Ireton et al., 2002). The pRS vector was a gift from Reuven Agami (The Netherlands Cancer Institute, Amsterdam, Netherlands). pRS human p120 siRNA and pRS m siRNA were generated according to Brummelkamp et al. (2002). In brief, a 64-bp linker was inserted into pRS using the BamHI and HindIII sites. Oligos for the linker contained p120-specific sense and corresponding antisense sequences, flanking a 6-base hairpin, and were PAGE purified by Integrated DNA Technologies. pEGFP-C1 δ-catenin (Lu et al., 1999) was a gift from Qun Lu (East Carolina University, Greenville, NC). pEGFP-C2 ARVCF C11 (Waibler et al., 2001) was a gift from Anna Starzinski-Powitz (Johann Wolfgang Goethe-Universität, Frankfurt, Germany). pEGFP-C1 (CLONTECH Laboratories, Inc.) was used as a negative control in transfection experiments.
