Mouse hippocampal cortical neurons were cultured from newborn CD1 mice according to standard procedures (Zhang et al., 2013). In short, hippocampi or cortices were dissected, papain-digested, and triturated. The dissociated cortical neurons were plated on matrigel-coated glass coverslips, and maintained in Neurobasal-A (NBA) medium with B-27 supplement. No FBS was ever added into the mouse cortical cultures. The lentiviral infection if applicable was performed at DIV4, and ApoE treatment (10 µg/ml) was applied from DIV 7. Cultures were harvested on DIV12-14 for indicated biochemical assays.
