Human neurons (iN cells) were generated from H1 and iPS cells essentially as described (Zhang et al., 2013) with the following modifications (Fig. S1A): ES cells were detached with accutase and plated onto matrigel-coated 6-well plates (4×104 cells/well) on day −2. Lentiviruses expressing Ngn2 and rtTA were prepared as described below, and added to the ES cells in fresh mTeSR1 medium (Stem Cell Technologies) on day −1. Doxycycline (2 mg/l, to activate Ngn2 expression) was added on day 0 (D0) in DMEM-F12 medium with N2 supplement without morphogens. Puromycin (1 mg/l) was added on D1 in fresh DMEM-F12/N2 + doxycycline medium for selection up to 24 hours. On D2, differentiating neurons were detached with accutase and re-plated on cultured mouse glia, MEFs, or just matrigel-coated 24-well plates (200,000 cells/well), and maintained in NBA/B-27 medium with no doxycycline. Various lentiviral infection of neurons were performed on D4 as described below; ApoE incubations were initiated on D10 and lasted until neurons were analyzed for different parameters. For mRNA and protein measurements, the assays were performed on D12 after ApoE treatments of 2
