of neurons were performed on D4 as described below; ApoE incubations were initiated on D10 and lasted until neurons were analyzed for different parameters. For mRNA and protein measurements, the assays were performed on D12 after ApoE treatments of 2 days, unless otherwise specified in time course studies. For immunoblotting analysis of protein phosphorylation, the ApoE was administered for 2 hours, and neuronal cultures were harvested immediately.
