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Chunk #5 — Materials and methods — Dataset for splicing model development

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RNA alternative splicing impacts the risk for alcohol use disorder.
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yes

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RNA sequencing data from the dorsolateral prefrontal cortex (DLPFC) and DNA genotyping data from 991 samples were downloaded from the CommonMind Consortium (CMC) [21]. The RNA-seq data were processed as described previously [26]. Genetic variants that had minor allele frequency (MAF) ≥ 0.03, Hardy–Weinberg equilibrium P > 0.001, and genotyping rate ≥0.95, were used as the input for imputation using the Michigan Imputation Server with default parameters. The reference panel used was 1000 G Phase 3 v5 (GRCh37.p13, hg19; EUR). EAGLE was used to phase genotypes and Minimac4 (v1.2.1) was used for imputation [27].