To determine statistical significance (p≤0.05) in studies comparing two or more groups, t tests (Prism, GraphPad, San Diego, CA, USA), one- and two-way ANOVA (JMP, SAS Institute, Cary, NC) followed by Bonferroni multiple comparison tests were performed as appropriate. Data from qRT-PCR studies in Figure 2B were analyzed by one-sample t tests (Prism) to individually compare Clic mRNA levels in mutants and revertants to control (100%).
