Young-adult age-matched worms, reared at 20°C, were placed on unseeded plates containing 0 mM or 400 mM ethanol (prepared as for ethanol response assays) for 5, 10 or 30 minutes. Ethanol-exposed worms were frozen at −80°C and homogenized in H2O. Internal ethanol was determined in the homogenates using a commercially available alcohol reagent (Pointe Scientific) and by calculating the volume of worms. Additional details are provided as Supplementary Materials.
