C. elegans were cultured according to Brenner (Brenner, 1974). Strains used in this study (N2 control and Clic mutant strains exc-4 (rh133) and exl-1(ok857)) were obtained from the C. elegans Genetics Center, which is funded by NIH-NCRR. The double mutant exc-4(rh133);exl-1(ok857) was generated by standard genetic crosses, using the recessive excretory canal phenotype that is associated with exc-4(rh133) to detect that mutation and a set of PCR primers to detect the exl-1(ok857) deletion mutation (5'-GTGCAATCTCGTCAGGACCAGGC-3', 5'-ATGCGTTACGATGCCCCGACAC-3'). Ethanol response assays were carried out as previously described (Davies et al., 2004, Davies et al., 2003) except additional time points were measured and ImagePro Plus was used for object tracking. Additional details are provided as Supplementary Materials.
