DE gene targets of the ethanol-responsive microRNAs were identified for each time point. Both experimentally validated and predicted targets (see methods) were utilized. The microRNAs changed at the 0h time point had a number of mRNA targets that were changed at the 0, 8 and 120h time points (Fig 4). In general, microRNAs that were changed at 8 or 120h had fewer targets than those changed at 0h, although NAC showed a number of targets for the microRNAs that were changed at 8h. For each brain region, the proportion of shared 0h microRNA targets was greatest (18–22%) for 0 and 8h (proportion calculated as the number of shared targets divided by the total number of targets in the earlier time point x100; data not shown). Most of the targets of microRNAs were dependent on the time point, however, there were some targets of microRNAs from the 0h point that were also changed at 0, 8 and 120h, or at 0 and 120 h (Table 3). There was little overlap between 8h microRNA targets at 8 and 120h (AMY: 4%, NAC: 7%, PFC: 0%).
