MicroRNAs were profiled in AMY, NAC and PFC at three different time points (0, 8 and 120h) after ethanol vapor treatment. After preprocessing, both the number of detected (395–419) and DE (42–47) microRNA probesets was similar within each brain region. Despite most of the detected microRNAs being expressed in all brain regions, the DE probes were distinct for each brain region (Fig 2). There was little overlap of DE microRNAs across time within each brain region (S1 Fig). MicroRNAs that were uniquely detected and DE in each brain region are shown in Table 1. Only three microRNAs were DE in all three brain regions (Table 2). MicroRNAs and mRNAs exhibited different temporal patterns of dysregulation (Fig 3). AMY and NAC showed very similar temporal profiles with the number of DE mRNAs decreasing over time, while DE microRNAs decreased from 0 to 8 hours and then rebounded at 120h. The temporal pattern of expression was unique in PFC because the number of DE microRNAs decreased as a function of time, and the number of DE mRNAs peaked at 8h, then declined at 120h.
