For chronic ethanol treatment of Aldh2−/−Fancd2−/−Trp53−/− and control mice, ethanol was administered in drinking water for ten days as reported previously6. For the first five days, the drinking water supply was replaced by a solution of 10:15:75 blackcurrant Ribena:ethanol:water, followed by a 10:20:80 solution for the last five days. A 50 μl blood sample was taken from tail veins before alcohol exposure, and by cardiac puncture at the end of the experiment, to measure full blood counts. Femurs were dissected for histological analysis and to determine bone marrow cellularity.
