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Chunk #12 — Materials and Methods — Drosophila behavioral experiments

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Glypican Gene GPC5 Participates in the Behavioral Response to Ethanol: Evidence from Humans, Mice, and Fruit Flies.
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Drosophila strains were maintained on standard cornmeal/molasses/yeast media at 25°C and 70% humidity with an approximately 16-hr/8-hr light/dark schedule. All strains were outcrossed for five generations to the Berlin genetic background strain carrying the w1118 eye-color marker. Strain sources were dallyMB950 (MB00950: Bloomington Stock Center), dally80 (Xinhua Lin), dlpf03537 (Exelixis/Harvard), and dlp1 (Scott Selleck).