Mice were injected (i.p.) with various doses of diazepam, THC, or vehicle and then tested in the marble burying assay. Locomotor activity was simultaneously recorded and was quantified as seconds per minute spent immobile. As reported previously, diazepam dose-dependently reduced marble burying [F(3,28) = 37.3; p < 0.0001; Figure 1A]. Post hoc analyses revealed that marble burying was decreased at 1 and 3 mg/kg doses. However, the 3 mg/kg dose also caused a significant increase in immobility, indicating sedation [F(3,532) = 18.6; p < 0.0001; Figure 1B]. Overall immobility increased over time during testing [F(19,532) = 3.8; p < 0.0001].
